Mouse KYNU / Kynureninase Protein (His Tag)

4432411A05Rik

Catalog Number	P50310-M08B
Organism Species	Mouse
Host	Baculovirus-Insect Cells
Synonyms	4432411A05Rik
Molecular Weight	The recombinant mouse KYNU consists of 474 amino acids and has a calculated molecular mass of 53.7 kDa. It migrates as an approximately 47 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 94 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse KYNU (Q9CXF0) (Met 1-Ser 464) was expressed，with a C-terminal polyhistidine tag.
Bio-activity	Measured by its ability to oxidize 3-hydroxykynurenine. The specific activity is > 300 pmoles/min/μg.
Research Area	Neuroscience \|Cell Type Marker in Neurodevelopment \|Neuronal Cell Markers \|Synapse & Synaptic Proteins \|Postsynaptic Density Protein
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Lima S, et al. (2007) Crystal structure of Homo sapiens kynureninase. Biochemistry. 46(10): 2735-44. Momany C, et al. (2004) Three-dimensional structure of kynureninase from Pseudomonas fluorescens. Biochemistry. 43(5): 1193-203. Toma S, et al. (1997) Cloning and recombinant expression of rat and human kynureninase. FEBS Lett. 408(1): 5-10.