Mouse IFNA14 / Interferon alpha-14 Protein (His Tag)

OTTMUSG00000007662

Catalog Number	P50669-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	OTTMUSG00000007662
Molecular Weight	The recombinant mouse IFNA14 comprises 177 amino acids and has a predicted molecular mass of 20.5 kDa. The apparent molecular mass of the protein is approximately 20 kDa in SDS-PAGE under reducing conditions.
predicted N	Cys 24
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mouse IFNA14 (NP_996858.1)(Cys24-Lys189) was expressed with a C-terminal polyhistidine tag.
Bio-activity	Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED₅₀ for this effect is typically 5-40 pg/mL.
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Inflammatory Cytokines & Chemoki and Receptors \|IL-10/Interferon Family
Formulation	Lyophilized from sterile PBS, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9. Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65. Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.