Mouse FCGRT & B2M Heterodimer Protein

FcRn

Catalog Number	PCT029-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	FcRn
Molecular Weight	The recombinant heterodimer of human FCGRT / B2M comprises 385 ( 285 + 100 ) amino acids and has a calculated molecular mass of 43.5 ( 31.8 + 11.7 ) kDa. The apparent molecular mass of rh FCGRT / B2M heterodimer is approximately 35 & 12 kDa respectively in SDS-PAGE under reducing conditions
predicted N	Ala 24 & Ile 21
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ser 297) of human FCGRT (P55899) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the human B2M (P61769) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified
Bio-activity	Measured by its ability to bind human IgG1 in functional ELISA.
Research Area	Immunology \|Innate Immunity \|Fc Receptor
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference