Mouse EGFR / HER1 / ErbB1 Protein (His Tag)

9030024J15Rik,AI552599,Erbb,Errb1,Errp,wa-2,wa2,Wa5

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Catalog Number	P51091-M08H
Organism Species	Mouse
Host	Human Cells
Synonyms	9030024J15Rik,AI552599,Erbb,Errb1,Errp,wa-2,wa2,Wa5
Molecular Weight	The secreted recombinant mouse EGFR comprises 634 amino acids and has a calculated molecular mass of 71 kDa. As a result of glycosylation, the apparent molecular mass of rmEGFR is approximately 100 kDa in SDS-PAGE under reducing conditions.
predicted N	Leu 25
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  human EGF-Fc (P10605-H01H), The EC50 of human EGF-Fc (P10605-H01H) is 60-90 ng/mL. 3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  mouse EGF-Fc (P50482-M01H), The EC50 of mouse EGF-Fc (P50482-M01H) is 70-100 ng/mL.
Research Area	Signaling |Signal Transduction |Protein Phosphorylation |Tyrosine Kinase |Receptor Tyrosine Kinases
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
Reference	Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25. Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48. Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.