Influenza A H7N9 (A/Hangzhou/1/2013) Hemagglutinin / HA Protein (His Tag)

HA

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Catalog Number	P40105-V08B
Organism Species	H7N9
Host	Baculovirus-Insect Cells
Synonyms	HA
Molecular Weight	The recombinant hemagglutinin of Influenza A virus (A/Hangzhou/1/2013 (H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa. The apparent molecular mass of the protein is approximately 59 kDa in SDS-PAGE under reducing conditions.
predicted N	Asp 19
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the Influenza A virus (A/Hangzhou/1/2013 (H7N9)) (AGI60301.1) hemagglutinin (Met1-Val524) was expressed with a C-terminal polyhistidine tag.
Bio-activity	1. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.6 μg/mL for 1% GRBC. 2. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37