Influenza A H3N2 (A/Brisbane/10/2007) Hemagglutinin Protein (HA1 Subunit) (His Tag)

HA1

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Catalog Number	P11056-V08H1
Organism Species	H3N2
Host	Human Cells
Synonyms	HA1
Molecular Weight	The recombinant HA1 subunit of Influenza A virus H3N2 (A/Brisbane/10/2007 (H3N2)) hemagglutinin comprises 340 amino acids with the predicted molecular mass of 38 kDa. As a result of glycosylation, it migrates as an approximately 60-65 kDa band in SDS-PAGE under reducing conditions.
predicted N	Gln 17
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the N-terminal segment of Influenza A virus H3N2 (A/Brisbane/10/2007 (H3N2)) hemagglutinin (ABW23353.1) (Met 1-Arg 345), termed as HA1, was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37