Influenza A H1N1 (A/California/04/2009) Hemagglutinin / HA Protein (His Tag)

HA

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Catalog Number	P11055-V08H2
Organism Species	H1N1
Host	Human Cells
Synonyms	HA
Molecular Weight	The secreted recombinant Influenza A virus (A/California/04/2009 (H1N1)) HA (mutated) comprises 527 amino acids and predicts a molecular mass of 60 kDa. As a result of glycosylation and proteolysis, the apparent molecular mass of the hemagglutinin is apparoximately 25-30, 50-55 and 80-85 kDa in SDS-PAGE under reducing conditions, corresponding to the HA2, HA1 subunit and the full length HA respectively.
predicted N	Asp 18
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain of Influenza A virus (A/California/04/2009 (H1N1)) hemagglutinin (ACP41105.1) (Met 1-Gln 529) with cleavage site mutated (IQS→RQRRRRK, HA1+HA2, partially cleaved) was expressed, with a C-terminal polyhistidine tag.
Bio-activity
Research Area	Microbiology |Pathogenic microorganism |viruses |animal virus |viral illness |Viral tract respiratory illness |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	White JM, Hoffman LR, Arevalo JH, et al. (1997). "Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin". In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses. Suzuki Y (March 2005). "Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses". Biol. Pharm. Bull. 28 (3): 399–408. Senne DA, Panigrahy B, Kawaoka Y, et al. (1996). "Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential". Avian Dis. 40 (2): 425–37