Human Semaphorin 4D / SEMA4D / CD100 Protein (His Tag)

C9orf164,CD100,coll-4,M-sema-G,SEMAJ

Catalog Number	P11825-H08H
Organism Species	Human
Host	Human Cells
Synonyms	C9orf164,CD100,coll-4,M-sema-G,SEMAJ
Molecular Weight	The recombinant human SEMA4D consists of 725 amino acids and predictes a molecular mass of 80.7 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSEMA4D is approximately 110 kDa due to glycosylation.
predicted N	Met 22
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human SEMA4D isoform 1 (Q92854-1) extracellular domain (Met 1-Arg 734) was expressed, with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized human SEMA4D-His at 10 μg/ml (100 μl/well) can bind mouse PTPRC-Fc (P50713-M02H1) with a linear range of 0.625-5 μg/ml.
Research Area	Neuroscience \|Neurology process \|Growth and Development \|Axon Guidance \|Semaphorin, Plexin, & Neuropilin
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference