Human R-Spondin 1 / RSPO1 Protein

CRISTIN3,R-Spondin 1,RSPO

100ug (NPP1582) Please inquiry

Catalog Number	P11083-HNAS
Organism Species	Human
Host	CHO Stable Cells
Synonyms	CRISTIN3,R-Spondin 1,RSPO
Molecular Weight	The recombinant human RSPO1 consists of 243 amino acids and has a predicted molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of it is approximately 40 and 31 kDa in SDS-PAGE under reducing conditions.
predicted N	Ser 21
SDS-PAGE
Purity	(53.2+43.4) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human RSPO1 (NP_001033722.1) ( Met 1-Ala 263 )was expressed and purified.
Bio-activity	Measured by its ability to induce activation of ßcatenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically 40-400 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
Research Area	Signaling \|Signal Transduction \|Growth Factor & Receptor \|R-Spondin Protein
Formulation	Lyophilized from sterile PBS, PH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	RSPO1 gene is a member of the R-spondin family. It encodes RSPO1 which is known as a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain. In mice, RSPO1 induces the rapid onset of crypt cell proliferation and increases intestinal epithelial healing, providing a protective effect against chemotherapy-induced adverse effects. This protein is an activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. RSPO1 acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. It also acts as a ligand for frizzled FZD8 and LRP6.
Reference	Kamata T, et al. (2004) R-spondin, a novel gene with thrombospondin type 1 domain, was expressed in the dorsal neural tube and affected in Wnts mutants. Biochim Biophys Acta. 1676(1):51-62. Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36(1):40-5. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.