Human PTH2 / PTRH2 Protein (His Tag)

PTRH2, BIT1, PTH2

Catalog Number	P13645-H07E
Organism Species	Human
Host	E. coli
Synonyms	PTRH2, BIT1, PTH2
Molecular Weight	The recombinant human PTRH2 consists of 132 amino acids and predicts a molecular mass of 14.5 KDa. It migrates as an approximately 14 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PTRH2 (Q9Y3E5) (Gly63-Tyr179) was expressed with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Immunology \|Signal Transduction \|Metabolism \|Pathways and Processes \|Metabolism processes \|Apoptosis \|
Formulation	Lyophilized from sterile 20mM Tris, 10% glycerol, pH 8.0. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	PTH2, also known as PTRH2, is a mitochondrial protein which belongs to the PTH2 family. PTH 2 is released during apoptosis from the mitochondria to the cytoplasm. The natural substrate for PTH 2 may be peptidyl-tRNAs which drop off the ribosome during protein synthesis. When in the cytoplasm, PTRH2 regulates the function of 2 transcriptional regulators, TLE5 and TLE1, thus promoting caspase-independent cell death.
Reference	Prahalad AK. et al., 2006, Proteomics. 6 (12): 3482-93. Jin L. et al., 2000, J Biol Chem. 275 (35): 27238-44. Boone T. et al., 2000, Nat Struct Biol. 7 (6): 486-91.