Human PI3 / Elafin / WFDC14 Protein (His Tag)
cementoin,ESI,MGC13613,SKALP,WAP3,WFDC14
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| Catalog Number | P12187-H08H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | cementoin,ESI,MGC13613,SKALP,WAP3,WFDC14 |
| Molecular Weight | The recombinant human PI3 consists of 106 amino acids and has a predicted molecular mass of 11.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh PI3 is approximately 13-16 kDa. |
| predicted N | Ala 23 |
| SDS-PAGE |  |
| Purity | > 92 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the human PI3 (NP_002629.1) (Met 1-Gln 117) was fused with a polyhistidine tag at the C-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |RNAi |Argonaute and Piwi |
| Formulation | Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | Elafin, also known as Elastase-specific inhibitor, Peptidase inhibitor 3, Protease inhibitor WAP3, Skin-derived antileukoproteinase, WAP four-disulfide core domain protein 14, PI3, WAP3 and WFDC14, is a secreted protein which contains one WAP domain. Elafin / PI3 consists of two domains: the transglutaminase substrate domain (cementoin moiety) and the elastase inhibitor domain. The transglutaminase substrate domain at the N-terminus serves as an anchor to localize elafin covalently to specific sites on extracellular matrix proteins. The serine anti-protease Elafin / PI3 is expressed by monocytes, alveolar macrophages, neutrophils, and at mucosal surfaces and possesses antimicrobial activity. It is also known to reduce lipopolysaccharide-induced neutrophil influx into murine alveoli as well as to abrogate lipopolysaccharide-induced production of matrix metalloprotease 9, macrophage inhibitory protein 2, and tumor necrosis factor-alpha by as-yet unidentified mechanisms. Elafin / PI3 is a neutrophil serine protease inhibitor expressed in lung and displaying anti-inflammatory and anti-bacterial properties. Elafin / PI3 is a neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Elafin / PI3 will regulate proteolytic enzymes during menstruation and will contribute to the innate defense against uterine infection. |
| Reference |