Human PGD / Phosphogluconate dehydrogenase Protein (His Tag)

6PGD

Catalog Number	P10393-H07E
Organism Species	Human
Host	E. coli
Synonyms	6PGD
Molecular Weight	The recombinant human PGD consisting of 494 amino acids and has a calculated molecular mass of 54.6 kDa. It migrates as an approximately 46 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 88 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human PGD (P52209) (Met 1-Ala 483) was expressed, with a polyhistide tag at the N-terminus.
Bio-activity
Research Area	Cancer \|Signal transduction \|Metabolism \|Types of disease \|Metabolism in Cancer
Formulation	Lyophilized from sterile PBS, pH 8.0 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Baillet A, et al. (2011) Coupling of 6-phosphogluconate dehydrogenase with NADPH oxidase in neutrophils: Nox2 activity regulation by NADPH availability. FASEB J. 25(7): 2333-43. Rho HK, et al. (2005) Transcriptional regulation of mouse 6-phosphogluconate dehydrogenase by ADD1/SREBP1c. Biochem Biophys Res Commun. 332(1): 288-96. Broedel SE, et al. (1990). Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase. J Bacteriol. 172 (7): 4023-31. Adams MJ, et al. (1983). The three dimensional structure of sheep liver 6-phosphogluconate dehydrogenase at 2.6 A resolution. EMBO J. 2 (6): 1009-14.