Human PARVA / Actopaxin Protein (GST Tag)

CH-ILKBP,MXRA2

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Catalog Number	P13919-H09E
Organism Species	Human
Host	E. coli
Synonyms	CH-ILKBP,MXRA2
Molecular Weight	The recombinant human PARVA /GST chimera consists of 606 amino acids and has a predicted molecular mass of 69.4 kDa. It migrates as an approximately 69 KDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 60 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human PARVA (Q9NVD7-1) (Met1-Glu372) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Signaling |Signal Transduction |Cytoskeleton / ECM |Extracellular Matrix |Structures |Focal Adhesions |
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Actopaxin, also known as alpha-parvin, belongs to the parvin family. It is widely expressed, with highest levels in heart, skeletal muscle, kidney and liver. Actopaxin contains 2 CH (calponin-homology) domains and probably plays a role in the regulation of cell adhesion and cytoskeleton organization. It interacts with integrin-linked protein kinase and probably with actin and the LD1 and LD4 motifs of PXN. Actopaxin binds directly to both F-actin and paxillin LD1 and LD4 motifs. Actopaxin also exhibits robust focal adhesion localization in several cultured cell types but is not found along the length of the associated actin-rich stress fibers. It is absent from actin-rich cell-cell adherens junctions.
Reference	Korenbaum E, et al. (2002) Genomic organization and expression profile of the parvin family of focal adhesion proteins in mice and humans. Gene. 279(1):69-79. Nikolopoulos SN, et al. (2002) Molecular dissection of actopaxin-integrin-linked kinase-Paxillin interactions and their role in subcellular localization. J Biol Chem. 277(2): 1568-75. Tu Y, et al. (2001) A new focal adhesion protein that interacts with integrin-linked kinase and regulates cell adhesion and spreading. J Cell Biol. 153(3): 585-98.