Human NSE / ENO2 / Enolase 2 Protein (His Tag)

HEL-S-279,NSE

Catalog Number	P13219-H08E
Organism Species	Human
Host	E. coli
Synonyms	HEL-S-279,NSE
Molecular Weight	The recombinant human ENO2 comprises 444 amino acids and has a calculated molecular mass of 48.6KDa. It migrates as an approximately 48 kDa band as predicted in SDS-PAGE under reducing conditions.
predicted N	Met 1
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human ENO2 (P09104) (Met 1-Leu 434) was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Cancer \|Oncoprotein & suppressor & biomarker \|Tumor biomarker \|Enzyme \|Neuron Specific Enolase
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Pechumer H, et al. (1994) Detection of neuron-specific gamma-enolase messenger ribonucleic acid in normal human leukocytes by polymerase chain reaction amplification with nested primers. Lab Invest. 69 (6): 743-9. Craig SP, et al. (1991) Localisation of neurone-specific enolase (ENO2) to 12p13. Cytogenet Cell Genet. 54 (1-2): 71-3. Muley T, et al. (2003) Technical performance and diagnostic utility of the new Elecsys neuron-specific enolase enzyme immunoassay. Clin Chem Lab Med. 41 (1): 95-103.