Human NRAS Protein (His Tag)
NRAS, HRAS1
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| Catalog Number | P12073-H07E |
|---|---|
| Organism Species | Human |
| Host | E. coli |
| Synonyms | NRAS, HRAS1 |
| Molecular Weight | The recombinant human NRAS consists of 202 amino acids and predicts a molecular mass of 23 KDa. It migrates as an approximately 24 KDa band in SDS-PAGE under reducing conditions. |
| predicted N | His |
| SDS-PAGE |  |
| Purity | > 95 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the mature form of human NRAS (P01111) (Met1-Cys186) was expressed with a polyhistidine tag at the N-terminus. |
| Bio-activity | |
| Research Area | Epigenetics |DNA / RNA |RNA Processing |RNA splicing |
| Formulation | Lyophilized from sterile 20mM Tris, 0.1M NaCl, 10% Glycerol, pH 7.5. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | NRAS was discovered by researchers at the Institute of Cancer Research, funded by the Cancer Research Campaign (now Cancer Research UK). NRAS gene is a member of the Ras gene family. It is mapped on chromosome 1, and it is activated in HL60, a promyelocytic leukemia line. The mammalian ras gene family consists of the harvey and kirsten ras genes (HRAS and KRAS), an inactive pseudogene of each (c-Hras2 and c-Kras1) and the N-ras gene. They differ significantly only in the C-terminal 40 amino acids. These ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. The NRAS gene specifies two main transcripts of 2Kb and 4.3Kb. The difference between the two transcripts is a simple extension through the termination site of the 2Kb transcript. The NRAS gene consists of seven exons (-I, I, II, III, IV, V, VI). |
| Reference |