Human KLK-1 / Kallikrein-1 Protein (His Tag)

hK1,Klk6,KLKR

Catalog Number	P10407-H08H
Organism Species	Human
Host	Human Cells
Synonyms	hK1,Klk6,KLKR
Molecular Weight	The secreted recombinant human KLK1 is a monomeric protein after cleavage of the signal peptide. It consists of 251 amino acids and predictes a molecular mass of 28 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
predicted N	Ser 23
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human KLK1 (NP_002248.1) precursor (Met 1-Ser 262) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin (PFR-AMC). The specific activity is >1,500 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)
Research Area	Immunology \|Inflammation / Inflammatory Mediator \|Plasma Cascade Systems in Inflammation \|Fibrinolysis System
Formulation	Lyophilized from sterile PBS, pH 7.4 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference