Human HAGH / GLO2 / Glyoxalase II Protein (His Tag)

GLO2,GLX2,GLXII,HAGH1

Catalog Number	P12146-H08E
Organism Species	Human
Host	E. coli
Synonyms	GLO2,GLX2,GLXII,HAGH1
Molecular Weight	The recombinant human HAGH consisting of 270 amino acids and has a calculated molecular mass of 30.2 kDa. It migrates as an approximately 19 kDa band in SDS-PAGE under reducing conditions
predicted N	Met
SDS-PAGE
Purity	> 96 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human HAGH isoform 2 (Q16775-2) (Met 1-Asp 260) was fused with a polyhistide tag at the C-terminus
Bio-activity
Research Area	Signaling \|Signal Transduction \|Metabolism \|Types of disease \|Metabolism in Cancer
Formulation	Lyophilized from sterile 20mM Tris, 0.15 M NaCl, 10% glycerol, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Mulley JC, et al. (1987). New regional localisations for HAGH and PGP on human chromosome 16. Hum Genet 74 (4): 423-4. Rulli A, et al. (2001). Expression of glyoxalase I and II in normal and breast cancer tissues. Breast Cancer Res. Treat. 66 (1): 67-72. Cordell PA, et al. (2004). The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II. J Biol Chem. 279 (27): 28653-61.