Human Galectin-8 / LGALS8 Protein (GST Tag)

Gal-8,PCTA-1,PCTA1,Po66-CBP

Catalog Number	P10301-H09E
Organism Species	Human
Host	E. coli
Synonyms	Gal-8,PCTA-1,PCTA1,Po66-CBP
Molecular Weight	The recombinant human LGALS8/GST chimera consists of 550 amino acids and predicts a molecular mass of 62.7 kDa. The apparent molecular mass of rh LGALS8/GST is approximately 60 kDa in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 76 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding human LGALS8 (AAF19370.1) (Met 1-Trp 317) was fused with the GST tag at the N-terminus.
Bio-activity	Measured by its ability to agglutinate human red blood cells. The ED₅₀ for this effect is typically 0.2-1 μg/ml.
Research Area	Cancer \|Signal transduction \|Cytoskeleton / ECM \|Cell Adhesion \|Lectin \|Galectin \|
Formulation	Lyophilized from sterile 50mM Tris, 0.1 M NaCl, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Levy Y, et al. (2003) Sustained induction of ERK, protein kinase B, and p70 S6 kinase regulates cell spreading and formation of F-actin microspikes upon ligation of integrins by galectin-8, a mammalian lectin. J Biol Chem. 278(16):14533-42. Nishi N, et al. (2003) Galectin-8 modulates neutrophil function via interaction with integrin alphaM. Glycobiology. 13(11):755-63. Bidon-Wagner N, et al. (2004) Human galectin-8 isoforms and cancer. Glycoconj J. 19(7-9):557-63.