Human CMA1 / Chymase 1 Protein (His Tag)

chymase,CYH,MCT1

Catalog Number	P11482-H08B
Organism Species	Human
Host	Baculovirus-Insect Cells
Synonyms	chymase,CYH,MCT1
Molecular Weight	The secreted recombinant human CMA1 consists of 238 amino acids and predicts a molecular mass of 26.6 kDa. It migrates as an approximately 33 kDa band in SDS-PAGE under reducing conditions.
predicted N	Gly 20
SDS-PAGE
Purity	> 92 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human CMA1 (P23946) (Met 1-Asn 247) was fused with a polyhistidine tag at the C-terminus.
Bio-activity
Research Area	Cardiovascular \|Blood \|Other in Blood
Formulation	Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Takai S, et al. (2003) Effect of chymase inhibitor on vascular proliferation. Jpn J Pharmacol. 90 (3): 223-7. Caughey GH, et al. (1993) The human mast cell chymase gene (CMA1): mapping to the cathepsin G/granzyme gene cluster and lineage-restricted expression. Genomics. 15 (3): 614-20. Urata H, et al. (1991) Cloning of the gene and cDNA for human heart chymase. J Biol Chem. 266 (26): 17173-9.