Human CD32a / FCGR2A Protein (167 Arg, His Tag)

CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2

Catalog Number	P10374-H08H
Organism Species	Human
Host	Human Cells
Synonyms	CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
Molecular Weight	The recombinant human CD32a consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
predicted N	Gln 34
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) precursor was expressed, with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.
Bio-activity	Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 12.5-200 ng/ml.
Research Area	Immunology \|Adaptive Immunity \|B Cell \|B Cell CD Antigen
Formulation	Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference