Human CD32a / FCGR2A Protein (167 Arg, Fc Tag)
CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
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| Catalog Number | P10374-H02H |
|---|---|
| Organism Species | Human |
| Host | Human Cells |
| Synonyms | CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2 |
| Molecular Weight | The recombinant human CD32a/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 426 amino acids and predicts a molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rh CD32a/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions. |
| predicted N | Gln 34 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) was fused with the Fc region of human IgG1 at the C-terminus. It is identical to FCGR2A131H/R in the reference. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD32a-Fc (P10374-H02H) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml. |
| Research Area | Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen |
| Formulation | Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
| Reference |