Human CD32a / FCGR2A Protein (166 Arg, His Tag)
CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2
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| Catalog Number | P10374-H08B |
|---|---|
| Organism Species | Human |
| Host | Baculovirus-Insect Cells |
| Synonyms | CD32,CD32A,CDw32,Fc gamma RIIA,FCG2,FcGR,FCGR2,FCGR2A1,IGFR2 |
| Molecular Weight | The recombinant human CD32a consists of 192 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 25 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. |
| predicted N | Gln 34 |
| SDS-PAGE |  |
| Purity | > 90 % as determined by SDS-PAGE |
| Protein Construction | A DNA sequence encoding the extracellular domain (Met 1-Gly 216) of human CD32a (AAH19931.1) precursor was expressed, with a C-terminal polyhistidine tag. |
| Bio-activity | Measured by its binding ability in a functional ELISA. Immobilized human CD32a (P10374-H08B) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.17 μg/ml. |
| Research Area | Immunology |Adaptive Immunity |B Cell |B Cell CD Antigen |
| Formulation | Lyophilized from sterile 20mM Tris, 50mM NaCl, pH 7.4 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. |
| Background | |
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