Human CASP3 / Caspase-3 / CASP-3 Protein (His Tag)

CPP32,CPP32B,SCA-1

Catalog Number	P10050-H08E
Organism Species	Human
Host	E. coli
Synonyms	CPP32,CPP32B,SCA-1
Molecular Weight	The recombinant human caspase3 consists of 259 amino acids and predicts a molecular mass of 29.9 KDa. It migrates as an approximately 17 and 15 KDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	(48.4+48.1) % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the mature form of human caspase3 (Q5IS54) (Ser29-His277) was expressed with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to cleave the fluorogenic peptide substrate Ac-DEVD-AFC. The specific activity is > 5000 pmoles/min/μg.
Research Area	Cancer \|Signal transduction \|Metabolism \|Pathways and Processes \|Metabolism processes \|Apoptosis \|
Formulation	Lyophilized from sterile 20mM HEPES, 100mM NaCl, 1mM EDTA, 10% Sucrose, 0.1% chaps, pH 7.5. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference