Human ARF5 / ADP-ribosylation factor 5 Protein (His Tag)

ARF5

Catalog Number	P14934-H07E
Organism Species	Human
Host	E. coli
Synonyms	ARF5
Molecular Weight	The recombinant human ARF5 consists of 198 amino acids and predicts a molecular mass of 22.8 KDa. It migrates as an approximately 23 KDa band in SDS-PAGE under reducing conditions.
predicted N	His
SDS-PAGE
Purity	> 95 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human ARF5 (AAP36805.1)(Met1-Arg180) was expressed with a polyhistidine tag at the N-terminus.
Bio-activity
Research Area	Immunology \|Signal Transduction \|Cytoskeleton / ECM \|Cytoskeletal Proteins \|Regulation
Formulation	Lyophilized from sterile PBS, 10% glycerol, pH 7.4. 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	ARF5, also known as ADP-ribosylation factor 5, belongs to the small GTPase superfamily, Arf family. Members of this family stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. ARF5 functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. ARF5 Is involved in protein trafficking. ARF5 may also modulate vesicle budding and uncoating within the Golgi apparatus.
Reference	Kanoh H. et al., 1997, J Biol Chem. 272 (9): 5421-9. Tsuchiya M. et al., 1991, J Biol Chem. 266 (5): 2772-7. McGuire RE. et al., 1997, Genomics. 41 (3): 481-4.