Human ANXA6 / Annexin A6 / Annexin6 Protein (His Tag)

ANX6,CBP68

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Catalog Number	P11161-H08E
Organism Species	Human
Host	E. coli
Synonyms	ANX6,CBP68
Molecular Weight	The recombinant human ANXA6 / Annexin A6 / Annexin6 consisting of 683 amino acids and has a calculated molecular mass of 77.2 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met 1
SDS-PAGE
Purity	> 97 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human ANXA6 / Annexin A6 / Annexin6 (P08133) (Met 1-Asp 673) was fused with a polyhistidine tag at the C-terminus.
Bio-activity	Measured by its ability to bind human CD21-His (P10811-H08H) in functional Elisa.
Research Area	Cardiovascular \|Blood \|Coagulation \|Coagulation Cascade
Formulation	Lyophilized from sterile PBS, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background	Annexin A6, also known as ANXA6 or ANXAⅥ, belongs to a family of Ca²⁺-dependent membrane and phospholipid binding proteins. Members of this family have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 6 is phosphorylated in vivo associated with cell growth. Annexin 6 was not phosphorylated in quiescent cells, but was phosphorylated on serine and to a lesser extent threonine, several hours following cell stimulation. Experiment has revealed the presence of annexin A6 on the cell surface of variety cells as putative receptors and / or binding proteins for chondroitin sulfate proteoglycans, helping cells to bind with this extracellular matrix glycosaminoglycan chondroitin sulfate which is related to the cell-substratum adhesion. A post-tranlational modification other than direct protein phosphorylation may influence the activity of annexin6 and provide evidence linking cell growth with regulation of annexin 6 function.
Reference	Takagi H, et al. (2002) Annexin 6 is a putative cell surface receptor for chondroitin sulfate chains. J Cell Sci. 115 (16): 3309-18. Moss SE, et al. (1992) A growth-dependent post-translational modification of annexin VI. Biochim Biophys Acta. 1160 (1): 120-6. Song G, et al. (1998) Altered cardiac annexin mRNA and protein levels in the left ventricle of patients with end-stage heart failure. J Mol Cell Cardio. 30 (3): 443-51.