Human 14-3-3 tau / 14-3-3 theta / YWHAQ Protein (GST Tag)

1C5,41701,HS1

Catalog Number	P10845-H09E
Organism Species	Human
Host	E. coli
Synonyms	1C5,41701,HS1
Molecular Weight	The recombinant human YWHAQ/GST chimera consists of 476 amino acids and has a predicted molecular mass of 54.6 kDa. It migrates as an approxiamtely 53 kDa band in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 88 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human YWHAQ (P27348) (Met 1-Asn 245) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Immunology \|Signal Transduction \|Other Related Intracellular Topics \|Apoptosis Adaptor Proteins
Formulation	Lyophilized from sterile 20mM Tris, 0.15m NaCl, 20mM GSH, pH 7.5 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Cheng Y, et al. (2010) Effect of 14-3-3 tau protein on differentiation in BeWo choriocarcinoma cells. Placenta. 31(1): 60-6. Umahara T, et al. (2010) 14-3-3 proteins and spinocerebellar ataxia type 1: from molecular interaction to human neuropathology. Cerebellum. 9(2): 183-9. Wang B, et al. (2004) A role for 14-3-3 tau in E2F1 stabilization and DNA damage-induced apoptosis. J Biol Chem. 279(52): 54140-52.