Human 14-3-3 eta / YWHAH Protein (GST Tag)

YWHA1

Catalog Number	P10847-H09E
Organism Species	Human
Host	E. coli
Synonyms	YWHA1
Molecular Weight	The recombinant human YWHAH/GST chimera consists of 476 amino acids and has a predicted molecular mass of 55 kDa as estimated in SDS-PAGE under reducing conditions.
predicted N	Met
SDS-PAGE
Purity	> 90 % as determined by SDS-PAGE
Protein Construction	A DNA sequence encoding the human YWHAH (Q04917) (Gly 2-Asn 246) was fused with the GST tag at the N-terminus.
Bio-activity
Research Area	Cancer \|Signal transduction \|Cellular Senescence and Pathways in Aging \|Apoptosis \|Death receptor & ligand \|Apoptosis Adaptor proteins \|
Formulation	Lyophilized from sterile 20mM Tris, 0.15M NaCl, 20mM GST, pH 8.0 1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
Background
Reference	Wakui H, et al. (1997) Interaction of the ligand-activated glucocorticoid receptor with the 14-3-3 eta protein. J Biol Chem. 272(13): 8153-6. Toyooka K, et al. (2002) Isolation and structure of the mouse 14-3-3 eta chain gene and the distribution of 14-3-3 eta mRNA in the mouse brain. Brain Res Mol Brain Res. 100(1-2): 13-20. Kim YS, et al. (2005) Role of 14-3-3 eta as a positive regulator of the glucocorticoid receptor transcriptional activation. Endocrinology. 146(7): 3133-40. Grover D, et al. (2009) Family-based association of YWHAH in psychotic bipolar disorder. Am J Med Genet B Neuropsychiatr Genet. 150B(7): 977-83.